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Image Search Results
Journal: Oncology Letters
Article Title: Artesunate inhibits proliferation and invasion of mouse hemangioendothelioma cells in vitro and of tumor growth in vivo
doi: 10.3892/ol.2017.6986
Figure Lengend Snippet: Transwell invasion assay using EOMA cells. The migration efficiency was illustrated as the increase or decrease in the number of invaded cells relative to the control. Cells from each sample were counted in three random fields at ×200 magnification (P<0.05). Results presented are representative data of ≥3 independent experiments.
Article Snippet: Matrigel-coated
Techniques: Transwell Invasion Assay, Migration
Journal: Bioengineered
Article Title: Circular RNA hsa_circ_0119412 contributes to tumorigenesis of gastric cancer via the regulation of the miR-1298-5p /zinc finger BED-type containing 3 (ZBED3) axis
doi: 10.1080/21655979.2022.2036406
Figure Lengend Snippet: Silencing hsa_circ_0119412 inhibits the survival and invasion of GC cells and promotes apoptosis (a) Relative expression of hsa_circ_0119412 was determined in MKN74 and GTL-16 cells transfected with si-circ (si-circ-1, si-circ-2) or si-NC by qRT-PCR. (b) The cell viability was measured in MKN74 and GTL-16 cells transfected with si-circ (si-circ-1, si-circ-2) or si-NC by CCK-8 assay. (c) The cell invasion ability was determined by transwell assay after knockdown of hsa_circ_0119412 (si-circ-1, si-circ-2) in MKN74 and GTL-16 cells. (d) The cell apoptosis rate was detected in MKN74 and GTL-16 cells after transfection with hsa_circ_0119412 siRNA plasmids (si-circ-1, si-circ-2) by flow cytometry assay. **P < 0.001 compared to si-NC. (e) Representative images of xenograft tumors of sh-circ and sh-NC group, tumor growth curves and tumor weight analysis were shown. (f) Relative expression of hsa_circ_0119412 was determined in xenograft tumors of sh-circ and sh-NC group. **P < 0.001 compared to sh-NC.
Article Snippet: This assay was conducted in a
Techniques: Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Transwell Assay, Knockdown, Flow Cytometry
Journal: Bioengineered
Article Title: Circular RNA hsa_circ_0119412 contributes to tumorigenesis of gastric cancer via the regulation of the miR-1298-5p /zinc finger BED-type containing 3 (ZBED3) axis
doi: 10.1080/21655979.2022.2036406
Figure Lengend Snippet: miR-1298-5p partially eliminated the functional effect of hsa_circ_0119412 on GC cells (a) Relative expression of miR-1298-5p was determined in MKN74 and GTL-16 cells transfected with si-circ or miR-1298-5p inhibitor by qRT-PCR. (b) The cell viability was measured in MKN74 and GTL-16 cells transfected with si-circ or miR-1298-5p inhibitor by CCK-8 assay. (c) The cell invasion ability was determined by transwell assay after knockdown of hsa_circ_0119412 or miR-1298-5p in MKN74 and GTL-16 cells. (d) The cell apoptosis rate was detected in MKN74 and GTL-16 cells after transfection with hsa_circ_0119412 siRNA or miR-1298-5p inhibito r plasmid s by flow cytometry assay. **P < 0.001 compared to si-NC; ^^P < 0.001 compared to inhibitor-NC; ##P < 0.001 compared to si-circ+inhibitor.
Article Snippet: This assay was conducted in a
Techniques: Functional Assay, Expressing, Transfection, Quantitative RT-PCR, CCK-8 Assay, Transwell Assay, Knockdown, Plasmid Preparation, Flow Cytometry
Journal: Bioengineered
Article Title: Circular RNA hsa_circ_0119412 contributes to tumorigenesis of gastric cancer via the regulation of the miR-1298-5p /zinc finger BED-type containing 3 (ZBED3) axis
doi: 10.1080/21655979.2022.2036406
Figure Lengend Snippet: miR-1298-5p plays a role in the proliferation, invasion and apoptosis of GC cells by negatively regulating ZBED3 level (a)Relative protein expression of ZBED3 was determined in MKN74 and GTL-16 cells transfected with si-ZBED3 or miR-1298-5p inhibitor by Western blot. (b) The cell viability was measured in MKN74 and GTL-16 cells transfected with si-ZBED3 or miR-1298-5p inhibitor by CCK-8 assay. (c) The cell invasion ability was determined by transwell assay after knockdown of ZBED3 or miR-1298-5p in MKN74 and GTL-16 cells. (d) The cell apoptosis rate was detected in MKN74 and GTL-16 cells after transfection with ZBED3 siRNA or miR-1298-5p inhibito r plasmid s by flow cytometry assay. **P < 0.001 compared to si-NC; ^^P < 0.001 compared to inhibitor-NC; ##P < 0.001 compared to si-ZBED3+ inhibitor.
Article Snippet: This assay was conducted in a
Techniques: Expressing, Transfection, Western Blot, CCK-8 Assay, Transwell Assay, Knockdown, Plasmid Preparation, Flow Cytometry
Journal: Bioengineered
Article Title: Circular RNA hsa_circ_0119412 contributes to tumorigenesis of gastric cancer via the regulation of the miR-1298-5p /zinc finger BED-type containing 3 (ZBED3) axis
doi: 10.1080/21655979.2022.2036406
Figure Lengend Snippet: Overexpression of ZBED3 worsened GC malignant phenotype and reversed the effect of hsa_circ_0119412 knockdown (a) Relative protein expression of ZBED3 was determined in MKN74 and GTL-16 cells transfected with si-circ or OE-ZBED3 by Western blot. (b) The cell viability was measured in MKN74 and GTL-16 cells transfected with si-circ or OE-ZBED3 by CCK-8 assay. (c) The cell invasion ability was determined by transwell assay in MKN74 and GTL-16 cells transfected with si-circ or OE-ZBED3. (d) The cell apoptosis rate was detected in MKN74 and GTL-16 cells after transfection with si-circ or OE-ZBED3 by flow cytometry assay. *P < 0.05, **P < 0.001 compared to si-NC; ^^P < 0.001 compared to OE-NC; #P < 0.05, ##P < 0.001 compared to si-circ+OE-ZBED3.
Article Snippet: This assay was conducted in a
Techniques: Over Expression, Knockdown, Expressing, Transfection, Western Blot, CCK-8 Assay, Transwell Assay, Flow Cytometry